🔬 Using a Microscope
Learn the parts of a compound microscope and how to bring the tiny world into sharp focus.
What you’ll learn
- Parts of a MicroscopeIdentify the main parts of a compound light microscope and say what each one does.A compound microscope uses two sets of lenses to magnify a sample lit from below. You look through the eyepiece while the objective lenses do most of the magnifying, the stage holds the slide, and the focus knobs sharpen the image. Knowing each part is the first step to using the microscope well.
- Preparing a SlidePrepare a slide and focus a microscope using the correct sequence of steps.A sample must be thin and flat on a glass slide so light can pass through it. To view it, place the slide on the stage, start with the lowest-power objective, use the coarse focus and then the fine focus to sharpen the image, and only then switch to a higher magnification. Turning the knobs gently keeps the lens from cracking the slide.
- The Power of MagnificationCalculate total magnification by multiplying the eyepiece magnification by the objective magnification.Magnification is how many times larger an object looks than its real size. A microscope's total magnification is found by multiplying the eyepiece number by the objective number, so a 10× eyepiece and a 40× objective give 400×. Higher magnification shows a smaller area in greater detail.
Questions this course answers
Which part of the microscope do you look through?
The eyepiece is the lens at the top that you look into, usually magnifying ten times.
Which part holds the slide in place?
The stage is the flat platform that the slide rests on while light shines up through it.
Which objective lens should you start with when viewing a slide?
You start with the lowest-power objective because it is easier to find and focus the sample before zooming in.
Which knob do you use last to make the image perfectly sharp?
The fine focus knob makes small, careful adjustments to sharpen the image after the coarse focus gets it roughly clear.
What is the total magnification of a 10× eyepiece and a 40× objective?
You multiply the two lenses together, so 10 × 40 = 400×.
How do you calculate a microscope's total magnification?
Total magnification is found by multiplying the eyepiece magnification by the objective magnification.
Grounded in trusted sources
- BBC Bitesize
- Britannica
- Khan Academy
- Britannica Kids
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