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📘 Read lanes as separate sample tracks

A gel image is a map of lanes, wells, and migration direction. Start by locating the wells, usually near the negative electrode, and the far end toward the positive electrode. Each vertical lane represents material loaded into one well: a D

3
lessons
~15 min
to learn
Adults
level
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What you’ll learn

  1. Orient the gelIdentify wells, lanes, ladder, migration direction, and image orientation.Smaller fragments usually migrate farther in a normally oriented agarose gel.
  2. Read the lanesCount bands, compare positions, and judge controls.Aligned bands suggest similar size, not identical sequence.
  3. Interpret the patternApply a stated comparison criterion and write a limited conclusion.Gel evidence is approximate size and pattern evidence, not full sequence proof.

Questions this course answers

What does a DNA ladder provide?

A ladder contains fragments of known lengths used to estimate sample band sizes.

In a normally oriented agarose gel, which fragments travel farther?

Smaller DNA fragments move through the gel pores more easily and migrate farther.

What does a matching band position most directly suggest?

Bands at the same migration position are consistent with similar length under the same run conditions.

What can a smear indicate?

A smear is diffuse signal rather than a neatly resolved set of discrete bands.

Why should a conclusion mention controls?

Positive and negative controls help establish whether the observed sample pattern supports a meaningful interpretation.

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