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🔬 How to Pipette Accurately

Choose the right micropipette, control aspiration and dispensing, select forward or reverse technique, and verify delivered volume with repeatable measurements.

lessons

~10 min

to learn

🔬 Science

subject

Adults

level

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What you’ll learn

Prepare the pipettePrepare a micropipette within its range with a compatible clean tip.Volume range, units, and a clean seal come before liquid handling.
Aspirate consistentlyCondition the tip and aspirate with controlled stops, depth, and speed.Consistent preparation and aspiration reduce bubbles and volume variation.
Dispense by the methodApply forward or reverse pipetting as the liquid and protocol require.The correct stop sequence depends on the chosen technique.
Protect and verifyPrevent carryover and test delivered volume with repeated measurements.Clean handling and evidence-based checks make technique trustworthy.

Questions this course answers

Which plunger position is normally used before aspirating in forward pipetting?

The first stop sets the intended air displacement; the second stop would draw extra liquid.

Why is the tip pre-wetted for many aqueous transfers?

Pre-wetting can reduce retention and evaporation effects at small volumes.

When can reverse pipetting be useful?

Reverse technique can improve handling of challenging liquids, when the method calls for it.

What does a gravimetric practice check help assess?

Repeated measured deliveries can be compared with the target and with one another.

What should happen after a tip contacts a sample?

Fresh tips help prevent contamination and carryover.

Grounded in trusted sources

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