Part 46

December 6: Weight, 9.90 kilos; 11.35 a. m., received 25 cc 2 per cent caffein solution by subcutaneous injection in back; 4 p. m., no symptoms, appetite good.

December 7-9: Received 25 cc caffein 2 per cent solution--subcutaneous injection, no symptoms, area of injection inflamed and swollen.

December 10, 13: Site of injection showed increased inflammation, received 0.495 gram caffein (50 mg per kilo) in 30 grams meat daily without showing any symptoms.

December 14: 12 noon, received 0.7425 gram caffein by mouth (0.075 per kilo); 2.30 p. m., restless and uneasy.

December 15: 11.30 a. m., received 0.7425 gram caffein by mouth; 2 p. m., depressed in spirits, although continues to have good appetite.

December 16: Weight, 9.50 kilos; 3.15 p. m., received 0.9509 gram caffein by mouth; 4.50 p. m., no symptoms.

December 17: Animal rested.

December 18: Received 0.950 gram caffein by mouth, no symptoms.

December 19: Received 0.9509 gram caffein by mouth, no symptoms.

December 20: 2.45 p. m., received 1.1875 grams caffein (0.125 gram per kilo); 4 p. m., restless and quite sick; ate only a little food.

December 21: 9 a. m., still uncomfortable, allowed to rest, no caffein given, gradually recovered appetite.

December 22: 11 a. m., received 1.875 grams caffein; 3 p. m., seemed sick, but showed no other symptoms, appetite fair.

December 23: 9 a. m., showed no symptoms from the day before, ate food gradually, seemed sick; 11.30 a. m., received 1.425 grams caffein (0.150 gram per kilo); 1.30 p. m., looked and behaved as if very sick, no other symptoms; 3.45 p. m., in attempting to get out of box fell over on back, had convulsions, whined, dyspnoea, died within 30 seconds.

Autopsy: Stomach exhibited mild inflammation; small intestine inflamed and hemorrhagic areas on mucosa; liver engorged and friable; spleen normal; kidneys slightly congested; other organs appeared normal. The total amount of caffein fed to Dog 29 was 12.135 grams, which was given in 18 days. The average daily amount per kilo was therefore 67.68 mg.

Dog 28. Male fox terrier.

Low nitrogen diet was begun about four weeks before the feeding of caffein; 50 mg of caffein was then fed for seven consecutive days. Partial loss of appetite was observed after the first dose. As the experiment progressed the desire for food steadily diminished, and the feces became fetid. Symptoms of intoxication manifested themselves early in the experiment, and vomiting occurred after the fourth dose. The dog was then put on a diet exclusively of meat. After an intermission of 10 days 109 mg caffein per kilo were given. Since there were no symptoms, the following day the amount was increased to 125 mg per kilo. This dose proved fatal within 16 to 20 hours.

This dog was stout and strong, weight 12.25 kilos, received daily 0.269 gram nitrogen per kilo (88.269 calories per kilo).

November 3: Weight, 11.75 kilos.

November 10: Weight, 11.95 kilos.

November 20: Weight, 11.20 kilos. All through this period had been kept in a cold, poorly ventilated room, put in a warm room, with bedding and good ventilation.

November 29: Weight, 11.95 kilos.

December 1: Put in a cage; weight, 11.95 kilos.

December 6: Weight, 11.95 kilos; 11.45 a. m., received 0.050 gram caffein per kilo; then received 30 cc 2 per cent caffein (0.6 gram) in practically one subcutaneous injection; 4.30 p. m., ate only part of food.

December 7: 10.25 a. m., received 30 cc 2 per cent caffein by subcutaneous injection (0.6 gram, or 50 mg, per kilo); 1.45 p. m., seemed sensitive to touch, no desire for food, depressed in spirit.

December 8: 11.40 a. m., received 30 cc 2 per cent caffein by subcutaneous injection (50 mg per kilo); 1 p. m., depressed in spirit, hind legs seemed somewhat stiff, no desire for regular food, site of injection inflamed.

December 9: 10.50 a. m., received 30 cc 2 per cent caffein by subcutaneous injection (50 mg per kilo); 2.30 p. m., had vomited, no desire for regular food.

December 10: Inflammation of site of injection, and swelling very pronounced; 2 p. m., received 0.5975 gram caffein, or 50 mg per kilo, with 30 grams of meat, refused regular food.

December 11, 12: Received 0.5975 gram caffein by mouth, no symptoms except refusal of regular food, feces fetid.