Part 25

April 1: 3.30 p. m., 2 cc 2 per cent caffein (138 mg per kilo) injected into peritoneal cavity; 5.35 p. m., symptoms present; reflexes much increased, but no tetanus. Survived.

SERIES B.

Guinea pig 51. Yellow female. Weight, 415 grams.

October 1: 9.50 a. m., 3 cc (144 mg per kilo) 2 per cent caffein injected into peritoneal cavity; 4.30 p. m., no symptoms, although under observation all day.

October 3: 2 p. m., alive.

Guinea pig 52. White male. Weight, 450 grams.

October 1: 9.45 a. m., 3.5 cc, 2 per cent caffein (155 mg per kilo), injected into peritoneal cavity; 4.30 p. m., no symptoms developed since injection.

October 3: 2 p. m., alive.

Guinea pig 58. Brown and white male. Weight, 490 grams.

October 1: 9.45 a. m., 4 cc, 2 per cent caffein (163 mg per kilo), injected into peritoneal cavity; 4.30 p. m., no symptoms developed since injection.

October 3: 2 p. m., alive.

October 8: Found dead. Autopsy: Congestion of lungs, spleen, liver, kidneys, and small intestines.

SERIES C.

Guinea pig 59. Gray and white. Weight, 375 grams. Diet, oats.

October 3: 2 p. m., 3.75 cc (0.2 gram per kilo) injected into peritoneal cavity; 2.15 p. m., reflexes increased but not markedly; 4 p. m., reflexes still more increased; no other symptoms; 5.30 p. m., no symptoms.

October 4: 8.50 a. m., guinea pig alive and active.

Guinea pig 58. Brown and white. Weight, 380 grams. Diet, oats.

October 3: 2 p. m., 3.8 cc caffein (0.2 gram per kilo), 2 per cent solution, injected into peritoneal cavity; 2.10 p. m., hind legs extended, then tetanus; attack lasted a few seconds, after which pig raised himself on his legs, but reflexes remained much exaggerated; 4 p. m. to 5.30 p. m., no symptoms of caffein intoxication.

October 4: 8.50 a. m., guinea pig alive and active.

Guinea pig 56. Gray and white male. Weight, 440 grams. Diet, oats.

October 1: 11.30 a. m., received 4.6 cc of 2 per cent caffein solution (0.2 gram per kilo) into abdominal cavity; 11.45 a. m., stiffness and rigidity of posterior extremities, reflexes increased; 12.30 p. m., hind legs paralyzed, reflexes increased; 4.35 p. m., no symptoms, guinea pig in good condition.

October 3: Still alive in good condition.

October 14: Died. Autopsy: Anterior lobe of right lung hepatized. Small portion of small intestine edematous. Other organs normal.

Guinea pig 55. White and yellow male. Weight, 690 grams. Diet, oats.

October 1: 11.30 a. m., received 6.5 cc of 2 per cent solution caffein (188 mg per kilo) into peritoneal cavity; 11.40 a. m., stiffness in all extremities, reflexes markedly increased; 12.30 p. m., reflexes increased, anterior and posterior extremities paralyzed; 3 p. m., found dead.

SERIES D.

Guinea pig 67. Gray and yellow male. Weight, 330 grams. Diet, oats.

October 5: 11.25 a. m., 4 cc of 2 per cent caffein injected into peritoneal cavity (240 mg per kilo); 11.30 a. m., tetanus--survived, convulsions off and on. Death at 2.55 p. m. Autopsy: Severe gastroenteritis; kidney petechiated; congestion of lungs and liver.