Part 13

March 1: 10.10 a. m., 8 cc 2 per cent caffein (0.1 gram per kilo) solution injected into peritoneal cavity; 10.40 a. m., rabbit urinated, reflexes increased, but no other symptoms; 10.50 a. m., no urine obtained from bladder; 2 p. m., 105 cc of clear pale urine collected; no albumen, no reduction; 3.40 p. m., no symptoms.

March 4: 2 p. m., rabbit looks well, urine collected, did not contain sugar.

Rabbit 298. Belgian hare, female. Weight, 1,205 grams. Diet, carrots.

March 1: 4.06 p. m., 7.5 cc 2 per cent caffein solution (0.125 gram per kilo) injected into peritoneal cavity, urine obtained from bladder immediately after injection, no albumen, no reduction; 5.30 p. m., reflexes increased, rabbit was able to run around, but became paralyzed soon; 5.40 p. m., rabbit is again able to run around.

March 3: 10 a. m., anterior extremities paralyzed, is able to use posterior extremities.

March 4: 1 p. m., rabbit looks normal.

Rabbit 223. Belgian hare, male. Weight, 1,165 grams. Diet, carrots.

March 1: 3.50 p. m., urine obtained from bladder clear, amber colored, no albumen, no sugar; 3.52 p. m., 7.5 cc 2 per cent caffein (125 mg per kilo) injected into peritoneal cavity; 5.40 p. m., rabbit makes little attempt to run when put on the floor, weakness of extremities marked.

March 4: 1.15 p. m., rabbit normal.

SERIES D.

The object of the experiments of this series was to study the effect of age on the resistance to caffein. Half-grown rabbits were, therefore, used in the following experiments.

Rabbit 310. Belgian hare, female. Weight, 880 grams. Diet, oats.

March 2: 3.25 p. m., 9 cc 2 per cent caffein (0.2 gram per kilo) injected into peritoneal cavity.

March 3: 9.30 a. m., no symptoms, rabbit looks normal.

March 4: 11 a. m., posterior extremities abducted, walked when placed on the floor, made no attempt to change attitude when placed on its side, remained some time in this position.

March 5: 9 a. m., found dead. Autopsy: Liver showed areas of degeneration; kidneys congestion and petechial hemorrhage on cortex; small and large intestines, inflammation marked; bladder distended.

Rabbit 75. Gray and white, female. Weight, 842 grams.

January 25: 3 p. m., 8.5 cc 2 per cent caffein solution (0.2 gram per kilo) injected into peritoneal cavity; 3.15 p. m., anterior extremities weak and reflexes increased.

January 27: Rabbit paralyzed but is able to turn over when placed on back.

Rabbit 74. Gray and white, female. Weight, 692 grams.

January 25: 3 p. m., 7 cc 2 per cent caffein (0.2 gram per kilo) solution injected into peritoneal cavity; 3.15 p. m., reflexes increased and anterior extremities paralyzed.

January 27: Rabbit recovered and is able to walk about in the room.

Rabbit 312, maltese, female. Weight, 740 grams. Diet, oats.

March 3: 11.47 a. m., urine obtained from bladder, appearance normal, no albumen, no reduction of Fehling's solution; 11.50 a. m., 7.5 cc 2 per cent caffein (0.2 gram per kilo) injected into peritoneal cavity; 2.30 p. m. anterior extremities paralyzed, posterior extremities rigid and extended; 5 p. m. (about), rabbit died.

Rabbit 311. Belgian hare, female. Weight, 650 grams. Diet, oats.

March 3: 11.26 a. m., urine obtained from bladder normal in appearance, albumen considerable, reduction of Fehling's solution none; 11.27 a. m., 6 cc 2 per cent caffein solution (0.2 gram per kilo) injected into peritoneal cavity; 2.30 p. m., rabbit seemed to be normal, no symptoms had developed; urine collected contained a large amount of sugar, reduction was very heavy, but no albumen was found.

March 4: 11 a. m., condition good, moves about when put on floor; gait, normal.

Rabbit 78. Yellow and white. Weight 659 grams.