Part 42

Foods, Their Composition and Analysis, p. 139.

Richardson, Vid. op. cit. 142, p. 158.

Principal Starches used as Food, Cirencester, Baily & Son, Market Place.

Vid. op. cit. 142, pp. 158 et seq.

Bulletin 44, Department of Agriculture, Division of Chemistry, p. 14.

PART THIRD.

PROCESSES FOR DETECTING AND DETERMINING SUGARS AND STARCHES AND OTHER CARBOHYDRATES IN CRUDE OR MANUFACTURED AGRICULTURAL PRODUCTS.

=203. Introduction.=—In the preceding part directions have been given for the estimation of sugars and starches in approximately pure forms. In the present part will be described the most approved methods of separating these bodies and other carbohydrates from crude agricultural products and for their chemical examination. In many respects the processes which in a small way are used for preparing samples for analysis are employed on a large scale for technical and manufacturing purposes. It is evident, however, that the following paragraphs must be confined strictly to the analytical side of the question inasmuch as anything more than mere references to technical processes would lead into wide digressions.

In the case of sugars the analyst is for the most part quite as much in need of reliable methods of extraction and preparation as of processes for analysis. With starches the matter is more simple and the chief methods of separating them for examination were necessarily described in the previous part.

Sugars in fresh plants exist almost entirely in solution. This is true of all the great sources of the sugar of commerce, viz., the palm, the maple, the sugar beet and sugar cane. This statement is also true of fruits and the natural nectar of flowers. By natural or artificial drying the sugar may be reduced to the solid or semisolid state as in the cases of raisins and honey. In certain seeds, deficient in water, sugars may possibly exist in a solid state naturally, as may be the case with sucrose in the peanut and raffinose in cotton seed.

Starches on the other hand when soluble, are probably not true starches, but they partake more or less of a dextrinoid nature. Fine starch particles occur abundantly in the juices of some plants, as for instance sorghum, where they are associated with sugar and can be obtained from the expressed juice by subsidence. But even in such a case it is not certain that the starch enters into the general circulation. It is more likely formed locally by biochemical condensation of its constituents. Starches in a soluble or semisoluble state are transported, as a rule, to the tubers or seeds of plants where they are accumulated in large quantities as a reserve food for future growth. For a study of the plant metabolism whereby starch is produced and for its histological and physiological properties the reader may consult the standard authorities on vegetable physiology.

=204. Sugar in the Sap of Trees.=—Many trees at certain seasons of the year, carry large quantities of sugar in their sap. Among these the maple and sugar palm are preeminent. The sap is secured by cutting a pocket into the side of the tree or by boring into it and allowing the sap to run into an appropriate receptacle through a spile. The content of sugar in the sap of the maple and palm varies greatly. In some cases it falls as low as one and a half and in others rises to as much as six or seven per cent. In most cases the sugar in the maple sap is pure sucrose, but towards the end of the flowing season it may undergo changes of a viscous nature due to fermentation, or inversion, forming traces of invert sugar. In this country the sap of the maple may flow freely on any warm day in winter, but the sugar season proper begins about February 15th in Southern Ohio and Indiana, and about March 25th in Vermont. It lasts from six weeks to two months. The sap flows best during moderately warm, still days, after a light freeze.

In addition to sugar the maple sap contains a trace of albuminoid matters and some malic acid combined with lime. As a rule it can be subjected to polarization without preliminary clarification.

=205. Determination of Sugar in Saps.=—In most cases the sap may be directly polarized in a 200 millimeter tube. Its specific gravity is obtained by a spindle or pyknometer, and the percentage of sugars taken directly from the table on page 73, the degree brix corresponding to the sugar percentage.

On polarizing, the sugar percentage is calculated as follows:

Multiply the specific gravity of the sap by 100 and divide the product by 26.048. Divide the direct reading of the sap on the sugar scale by the quotient obtained above, and the quotient thus obtained will be the correct percentage of sugar in the original solution.

The formula is applicable for those instruments in which 26.048 grams represent the normal quantity of sugar which in 100 cubic centimeters reads 100 divisions on the scale. When other factors are used they should be substituted for 26.048 in the above formula.

The principle of the calculation is based on the weight of the sap which is contained in 100 cubic centimeters, and this is evidently obtained by multiplying 100 by the specific gravity of the sap. Since 26.048 is the normal quantity of sugar in that volume of the solution the quotient of the actual weight divided by that factor shows how many times too great the observed polarization is. The simple division of the polariscope reading by this factor gives the correct reading.

Example: Let the specific gravity of the sap be 1.015 and the observed polarization be 15.0. Then the true percentage of sugar in the sap is found by the equation:

101.5 : 26.048 = 15.0 : x.

Whence x = 3.85 = percentage of sugar in the sap.

The process outlined above is not applicable when a clarifying reagent such as lead subacetate or alumina cream must be used. But even in these cases it will not be found necessary to weigh the sap. A sugar flask graduated at 100 and 110 cubic centimeters is used and filled to the first mark with the sap, the specific gravity of which is known. The clarifying reagent is added, the volume completed to the second mark with water, and the contents of the flask well shaken and thrown on a dry filter. The observation tube, which should be 220 millimeters in length, is then filled with the clear filtrate and the rest of the process is as described above. A 200 millimeter tube may also be used in this case and the observed reading increased by one-tenth.

=206. Estimation of Sugar in the Sap of Sugar Cane and Sorghum.=—In bodies like sugar cane and sorghum the sap containing the sugar will not flow as in the cases of the maple and sugar palm. The simplest way of securing the sap of the bodies named is to subject them to pressure between rolls. A convenient method of obtaining the sap or juice is by passing the cane through a small three-roll mill indicated in the figure. Small mills of this kind have been used in this division for many years and with entire satisfaction. Small canes, such as sorghum, may be milled one at a time, or even two or three when they are very small. In the case of large canes, it is necessary that they be split and only half of them used at once. The mill should not be crowded by the feed in such a way as to endanger it or make it too difficult for the laborer to turn. From fifty to sixty per cent of the weight of a cane in juice may be obtained by passing it through one of these small mills. Experience has shown that there is a little difference between the juice as first expressed and the residual sap remaining in the bagasse, but the juice first expressed may be used for analysis for control purposes as a fair representative of all that the cane contains.

To determine the percentage of juice expressed, the canes may be weighed before passing through the mill and the juice collected. Its weight divided by the weight of the original cane will give the per cent of the juice expressed, calculated on the whole cane. Instead of weighing the juice the bagasse may also be collected and weighed; but on account of the rapidity with which it dries the operation should be accomplished without delay. The expressed juice is clarified with lead subacetate, filtered and polarized in the manner described in former paragraphs. Instead of weighing the juice, its specific gravity may be taken by an accurate spindle and the volume of it, equivalent to a given weight, measured from a sucrose pipette.

A sucrose pipette for cane juice has a graduation on the upper part of the stem which enables the operator to deliver double the normal weight for the polariscope used, after having determined the density of the juice by means of a spindle. A graduation of from 5° to 25° of the brix spindle will be sufficient for all variations in the density of the juice, or one covering a range of from 10° to 20° will suffice for most instances. The greater the density of the juice the less volume of it will be required for the weight mentioned. For general use, the sucrose pipette is graduated on the stem to deliver from forty-eight to 50.5 cubic centimeters, the graduations being in terms of the brix spindle. The graduation of the stem of this instrument is shown in the accompanying figure. In the use of the pipette it is only necessary to fill it to the degree on the stem corresponding to the degree brix found in the preliminary trial.

The quantities of juice corresponding to each degree and fractional degree of the brix spindle are given in the following table; calculated for the normal weight 26.048 grams for the ventzke and for 16.19 grams for the laurent scale. The measured quantities of juice are placed in a 100 cubic centimeter sugar flask, treated with the proper quantity of lead subacetate, the volume completed to the mark, and the juice filtered and polarized in a 200 millimeter tube. The reading of the polariscope is divided by two for the factor 26.048 and by three for the factor 16.19.

TABLE FOR USE OF SUCROSE PIPETTES.

Cubic centimeters Cubic centimeters of juice for 26.048 of juice for 16.19 Degrees factor. Divide Degrees factor. Divide brix. reading by two. brix. reading by three.