Part 14

Everything depends on the proper management of the object and the arrangement of the light. Some opaque objects can be seen best by direct light, and others by transmitted light. If a leaf be examined, particularly if it be a thick and heavy one, like that of the ivy, the upper and lower membranes must be stripped apart--a task which is easily performed by tearing a small slit, and then ripping it smartly across. A pair of forceps will be required for this and other delicate work, and may be obtained at a cheap rate. Care must be taken to keep the points exactly even, and if at any time one of them appears to be shorter than the other, they should be rubbed on a hone until they are brought perfectly level.

These should be made of steel; but the young microscopist will find that a second pair, made of brass, and much rougher in finish, are invaluable aids as he takes his walks into the country. By their aid he can pick up minute objects, draw insects out of crevices without damaging them, and pluck the tiniest flowers without harming their petals. They can be carried in the waistcoat pocket, and the cost is sixpence. Any lad who knows how to handle solder can make a pair for himself in a few minutes.

A penknife with one blade kept scrupulously sharp is essential, and we have found an old lancet of the greatest service. Lancets have gone so much out of fashion, that the second-hand instrument shops abound with them. We did not allow our own lancet to be shut up, but removed the blade from the tortoise-shell handle, and fixed it upon a wooden handle, about four inches in length, so that it looked very clumsy, but was extremely useful.

Two pairs of scissors are needful,--one very fine and the other moderately strong. Both pairs, however, must have very short blades and very long handles, and the scissors such as ladies use are of very little use, the short handles causing the fingers of the right hand to shade the object. As to the fine pair, it is hardly possible to have the handles too long or the blades too short; for if the points can be separated a quarter of an inch, nothing more is needed. If a pair of bent scissors can also be obtained, they are extremely pleasant to work with, and save much trouble.

Pill-boxes of various sizes are of very great service to the microscopist. We always have them arranged in “nests,” i. e., six or seven inside each other, so that space is greatly economized, as long as they are not in absolute use. All delicate objects should be placed in separate boxes, and the predaceous insects must be treated in the same manner, or they will certainly destroy one another, or, at all events, inflict such injuries as will make them useless for microscopic purposes.

When the insects are to be killed on the spot, we employ another and a very simple plan.

We take one of the old-fashioned wooden lucifer-match boxes, bore a hole in the lid, and push through the hole a swan-quill, or the barrel of one of the swan-quill steel pens. A glass tube is still better, but is too fragile. Beeswax is tightly worked into the junction of the tube with the wood, so as to make it as nearly air-tight as possible. A cork stopper is then cut to fit the tube. When this is finished, we take the smallest-sized pill-box, bore a number of holes in it with a red-hot needle, place a little piece of solid ammonia within it, and inclose it in the lucifer-box. Its effects are almost instantaneous; for scarcely has the insect touched the bottom of the box before it is helpless, and in a very few moments it is quite dead, so powerful is ammonia towards insects. The reader will of course understand that the pill-boxes must never have been used for pills, and that the match-box must be carefully cleaned before employing it in the microscopic service. Moreover, any boxes that have been used for insects become useless, inasmuch as the scales always fall from the wings, and cling to the sides of the box, so as to mix with succeeding objects, and very much puzzle the observer.

Aquatic and marine objects require bottles, and, as a general rule, these bottles ought always to have wide mouths. Indeed, if there be no shoulder at all, their purpose will be better served, as a small object is very apt to be caught under the shoulder, and to give much trouble before it can be removed without injury. Wide and short test-tubes answer admirably for collecting; and it will always be advisable to have a few small test-tubes ready fitted with corks, for the purpose of isolating those specimens which might receive or cause injury by being mixed with others.

To remove minute objects from one vessel into another is a very easy process. Take a glass tube, mark off a portion about eight inches in length, cut a little notch with a file, and bend it smartly, when it will break neatly across, without leaving points or having the regularity of its ends injured by gaps. Turn each end round and round in the flame of the spirit-lamp, and you have an ordinary “pipette.” The object of placing the ends of the tube in the flame is to render the edges quite smooth and rounded.

Now mark off the same length of tube, and place the marked portion in the flame, taking care to warm it well first, lest the sudden heat should crack the glass. Keep it continually turning between the fingers, and when it is quite soft, and of a fine red heat, draw the hands smartly apart, and you will produce a couple of tubes tapering to very fine points. Break off the tapering portions at any convenient point, round the edges as before, and you will then have pipettes suitable for small objects. As there are many specimens, especially the smaller animalculæ, which have a habit of retiring into the remotest corner, it is necessary to bend another pipette, so as to follow them. For our own part, we prefer the pipette to be bent nearly to a right angle.

The mode of using these simple instruments is as follows:--Place the forefinger or thumb firmly on the large end, and push the point under water. When the opening is close to the sought-for object, lift the finger suddenly, and admit the air into the tube. The water will immediately rush in at the lower end, and if the orifice has been properly directed, will carry the object into the tube. The finger is again applied to the mouth of the tube, and the object can be then carried off.

As with the pocket-lens almost every object is to be viewed by means of direct light, the young observer will find himself much aided by a suitable background. Any small object, such as a minute insect, a seed, or a hair, becomes very indistinct if held up against the light, or even when viewed against a broken background of trees, houses, or herbage. The simplest plan of securing a proper background is to take a disc of ivory or even of white cardboard, and to blacken one side of it. The black paint which is used for this purpose must be without gloss, and have what is called a “dead” surface. Ink answers very well for the purpose, and so does ivory-black; but Indian ink is too glossy to be serviceable.

To procure specimens from the water is a matter of some difficulty if managed badly, but easy enough when the collector knows his business. It is of course needful to attach the collecting vessel to the end of a rod, and to plunge it into the spots which look most favorable. Now even so simple a matter as this requires some little care, if the young microscopist really wishes to obtain the best specimens. A common walking-stick will answer most purposes; but the most efficient rod for the purpose is one of the common walking-stick fishing-rods without the top joint, as it can be carried without attracting attention, and can be lengthened at will by adding the different joints.

Many methods have been proposed by which the vessel is to be attached to the rod; but that which I am about to describe is certainly the simplest and most effective that I have tried. Get a piece of gutta-percha tubing, just large enough to be slipped on the end of the rod or stick; mark off an inch or so, and cut the tube nearly through, then cut it away longitudinally, so that a long tongue of gutta-percha is left, and the instrument is completed.

Its application is as simple as its structure. Bend the tongue over, so as to form a loop, and push the end through the short tube. Slip the neck of the bottle into the loop, and draw the tongue until it is tolerably tight. Push the end of the stick into the tube, taking care to hold the tongue firmly in its place, and the vessel will then be fastened at right angles to the stick.

The method of collecting by means of this instrument is as follows: Immerse the vessel in the water, with the mouth downwards, so that no water may enter. Push it gently towards the spot which is to be investigated, move it about a little, so as to cause a disturbance, and then turn the vessel with its mouth upwards. Water will instantly rush in, carrying with it the objects which are to be examined. The contents of the vessel may then be transferred to the large bottle, and another dip made. Confervoid growths, especially those which accumulate in a kind of scum on the surface, should be obtained very quietly, without previous disturbance of the water.

After the pond, or stream, or ditch has been well searched, the bottle should be roughly examined, by means of a pocket-lens, and the contents sorted into the smaller tubes, as has already been mentioned. This precaution is especially needful when any of the minute crustacea called Entomostraca are captured, as they are most voracious beings, and will make sad havoc among other specimens, unless they are placed in separate bottles. They are mostly large enough to be detected with the naked eye, and look something like little fleas as they move along.

As the Entomostraca cast their shells repeatedly during their lives, some species performing this operation every two days, a beautiful series of objects can be obtained by gathering the cast shells and preparing them for the microscope, according to the directions that will be found in the following pages. These shells are peculiarly valuable, as they retain the chief external characteristics of the creature to which they belonged, the limbs, plumes, and even the delicate bristles being preserved entire. It is in the power of the microscopist to retard or hasten the change of shell, heat and light aiding development, and cold and darkness retarding it. The remarkable “ephippium,” or saddle, which is found on the backs of the Daphnia, the Moina, and other Entomostraca, and which is used as a receptacle for eggs, should be searched for and preserved.

A very thin and a very flat bottle is a most useful assistance in detecting the character of any unknown object, especially if it be living. Such a bottle may easily be made by heating one of the small test tubes in the spirit lamp until it is of a glowing red heat, and then pressing the sides together. Some little neatness is required in this process, as an unskillful operator is apt to press the sides unequally, and to leave a bulging projection at the end.

THE COMPOUND MICROSCOPE.

We have already described the simpler forms of magnifying instruments, together with the best method of using them. We now purpose to describe the more complicated instrument called the compound microscope, and hints will be given as to the best method of making preparations for it.

The great distinction between the simple and compound microscope is, that whereas the former instrument magnifies the object, the latter magnifies the magnified image of the object. In the least elaborate form of this instrument there are two glasses, one at each end of a tube, the small glass magnifying the object, and being therefore called the “object-glass,” while the other, which magnifies the image of the object, is placed next to the eye, and is therefore termed the “eye-glass.” In practice, however, this arrangement is found to be so extremely defective, that the instrument was quite useless except as an experimental toy; for the two enemies of the optician, chromatic and spherical aberration, prevailed so exceedingly, that every object appeared as if surrounded with prismatic colors, and every line was blurred and indistinct.

In this uncertain state the compound microscope remained for many years, its superb capabilities being scarcely recognized. The chief fault was thought to be in the material of which the object-glass was made, and for a long series of years all experiments were conducted with a view to an improvement in this respect. When, however, the diamond had been employed as an object-glass, and had failed equally with those of less costly material, attention was directed to the right point--namely, the arrangement of the different glasses,--and at length opticians succeeded in obtaining a pitch of excellence which can be almost termed perfection. It would be impossible to describe the method which is employed for this purpose, and it must suffice to say that the principle is that of playing off one defect against another, and so making them mutually correct their errors.

The magnifying powers of the compound microscope can be very great, and it is therefore necessary that extreme care should be taken in its manipulation. It will be possible for a clumsy person to do more damage to a good instrument in three minutes than can be repaired in as many weeks.

Before proceeding to the management of the microscope and the construction of the “slides,” we will briefly describe one or two chief forms of the compound microscope.

The simplest form of the compound microscope, as at present made, consists of a stand and a sliding tube, in which are set the glasses which magnify the object and its image. At the top is the tube, which is capable of being slid up and down in the shoulder of the stand, so as to obtain the proper focus. Above is seen the eye-glass; and the object-glass is shown at the bottom of the tube. Below the object-glass is the “stage” on which the object to be magnified is laid; and lowest of all is a mirror, which serves to reflect the light upwards through the object, and which can be turned by means of the knobs at the sides. The object-glass is composed of two pieces, which can readily be separated. If both are used, sufficient magnifying power is gained to show the scales on a butterfly’s wing and similar minute objects; while, if one is removed, the object is not magnified to so great an extent, but a larger portion can be seen, and the definition is clearer. The cost of this instrument, together with a few accessories, is about $2.50.

The proper light is our next point, and upon it rests the chief beauty of the effect. The light which will suit one object will not suit another, and even the same object should be examined under every variety of light. Some objects are best shown when the light is thrown upon them from above, and others when it is thrown through them from below. Again, the direction of light is of vast importance; for it will easily be seen that an oblique light will exhibit minute projections by throwing a shadow on one side and brilliancy on the other, while a vertical illumination would fail to show them. On the same principle, one object will be shown better with the light in front, and another when it is on one side.

One of the most effective means of attaining this object is by using the “bull’s-eye condenser,” which is sometimes fixed to the stage, but is usually detached. As the upright stem is telescopic, the glass can be raised to a considerable height, while the joint and sliding-rod permit the lens to be applied at any angle which promises the most brilliant light.