Part 21

But I do not wish to leave an assertion of this kind without illustration. Take, then, the well-conceived experiments of Dr. Hughes Bennett, described before the Royal Society of Surgeons in Edinburgh on January 17, 1868. [Footnote: 'British Medical Journal,' 13, pt. ii. 1868.] Into flasks containing decoctions of liquorice-root, hay, or tea, Dr. Bennett, by an ingenious method, forced air. The air was driven through two U-tubes, the one containing a solution of caustic potash, the other sulphuric acid. 'All the bent tubes were filled with fragments of pumice-stone to break up the air, so as to prevent the possibility of any germs passing through in the centre of bubbles.' The air also passed through a Liebig's bulb containing sulphuric acid, and also through a bulb containing gun-cotton.

It was only natural for Dr. Bennett to believe that his 'bent tubes' entirely cut off the germs. Previous to the observations just referred to, I also believed in their efficacy. But these observations destroy any such notion. The gun-cotton, moreover, will fail to arrest the whole of the floating matter, unless it is tightly packed, and there is no indication in Dr. Bennett's memoir that it was so packed. On the whole, I should infer, from the mere inspection of Dr. Bennett's apparatus, the very results which he has described--a retardation of the development of life, a total absence of it in some cases, and its presence in others.

In his first series of experiments, eight flasks were fed with sifted air, and five with common air. In ten or twelve days all the five had fungi in them; whilst it required from four to nine months to develop fungi in the others. In one of the eight, moreover, even after this interval no fungi appeared. In a second series of experiments there was a similar exception. In a third series the cork stoppers used in the first and second series were abandoned, and glass stoppers employed. Flasks containing decoctions of tea, beef, and hay were filled with common air, and other flasks with sifted air. In every one of the former fungi appeared and in not one of the latter. These experiments simply ruin the doctrine that Dr. Bennett finally espouses.

In all these negative cases, the prepared air was forced into the infusion when it was boiling hot. Dr. Bennett made a fourth series of experiments, in which, previous to forcing in the air, he permitted the flasks to cool. Into four bottles thus treated he forced prepared air, and after a time found fungi in all of them. What is his conclusion? Not that the boiling hot liquid, employed in his first experiments, had destroyed such germs as had run the gauntlet of his apparatus; but that air which, previous to being sealed up, had been exposed to a temperature of 212°, is too rare to support life. This conclusion is so remarkable that it ought to be stated in Dr. Bennett's own words. 'It may be easily conceived that air subjected to a boiling temperature is so expanded as scarcely to merit the name of air, and that it is more or less unfit for the purpose of sustaining animal or vegetable life.'

Now numerical data are attainable here, and as a matter of fact I live and flourish for a considerable portion of each year in a medium of less density than that which Dr. Bennett describes as scarcely meriting the name of air. The inhabitants of the higher Alpine chalets, with their flocks and herds, and the grasses which support these, do the same; while the chamois rears its kids in air rarer still. Insect life, moreover, is sometimes exhibited with monstrous prodigality at Alpine heights.

In a fifth series of experiments sixteen bottles were filled with infusions. Into four of them, while cold, ordinary unheated and unsifted air was pumped. In these four bottles fungi were developed. Into four other bottles, containing a boiling infusion, ordinary air was also pumped--no fungi were here developed. Into four other bottles containing an infusion which had been boiled and permitted to cool, sifted air was pumped--no fungi were developed. Finally, into four bottles containing a boiling infusion sifted air was pumped no fungi were developed. Only, therefore, in the four cases where the infusions were cold infusions, and the air ordinary air, did fungi appear.

Dr. Bennett does not draw from his experiments the conclusion to which they so obviously point. On them, on the contrary, he founds a defence of the doctrine of spontaneous generation, and a general theory of spontaneous development. So strongly was he impressed with the idea that the germs could not possibly pass through his potash and sulphuric acid tubes, that the appearance of fungi, even in a small minority of cases, where the air had been sent through these tubes, was to him conclusive evidence of the spontaneous origin of such fungi. And he accounts for the absence of life in many of his experiments by an hypothesis which will not bear a moment's examination. But, knowing that organic particles may pass unscathed through alkalies and acids, the results of Dr. Bennett are precisely what ought wider the circumstances to be expected. Indeed, their harmony with the conditions now revealed is a proof of the honesty and accuracy with which they were executed.

The caution exercised by Pasteur both in the execution of his experiments, and in the reasoning based upon them, is perfectly evident to those who, through the practice of severe experimental enquiry, have rendered themselves competent to judge of good experimental work. He found germs in the mercury used to isolate his air. He was never sure that they did not cling to the instruments he employed, or to his own person. Thus when he opened his hermetically sealed flasks upon the Mer de Glace, he had his eye upon the file used to detach the drawn-out necks of his bottles; and he was careful to stand to leeward when each flask was opened. Using these precautions, he found the glacier air incompetent, in nineteen cases out of twenty, to generate life; while similar flasks, opened amid the vegetation of the lowlands, were soon crowded with living things. M. Pouchet repeated Pasteur's experiments in the Pyrenees, adopting the precaution of holding his flasks above his head, and obtaining a different result. Now great care would be needed to render this procedure a real precaution. The luminous beam at once shows us its possible effect. Let smoking brown paper be placed at the open mouth of a glass shade, so that the smoke shall ascend and fill the shade. A beam sent through the shade forms a bright track through the smoke. When the closed fist is placed underneath the shade, a vertical wind of surprising violence, considering the small elevation of temperature, rises from the band, displacing by comparatively dark air the illuminated smoke. Unless special care were taken such a wind would rise from M. Pouchet's body as he held his flasks above his head, and thus the precaution of Pasteur, of not coming between the wind and the flask, would be annulled.

Let me now direct attention to another result of Pasteur, the cause and significance of which are at once revealed by the luminous beam. He prepared twenty one flasks, each containing a decoction of yeast, filtered and clear. He boiled the decoction so as to destroy whatever germs it might contain, and, while the space above the liquid was filled with pure steam, he sealed his flasks with a blow-pipe. He opened ten of them in the deep, damp caves of the Paris Observatory, and eleven of them in the courtyard of the establishment. Of the former, one only showed signs of life subsequently. In nine out of the ten flasks no organisms of any kind were developed. In all the others organisms speedily appeared.

Now here is an experiment conducted in Paris, on which we can throw obvious light in London. Causing our luminous beam to pass through a large flask filled with the air of this room, and charged with its germs and its dust, the beam is seen crossing the flask from side to side. But here is another similar flask, which cuts a clear gap out of the beam. It is filled with unfiltered air, and still no trace of the beam is visible. Why? By pure accident I stumbled on this flask in our apparatus room, where it had remained quiet for some time. Acting upon this obvious suggestion I set aside three other flasks, filled, in the first instance, with mote-laden air. They are now optically empty. Our former experiments proved that the life-producing particles attach themselves to the fibres of cotton-wool. In the present experiment the motes have been brought by gentle air-currents, established by slight differences of temperature within our closed vessels, into contact with the interior surface, to which they adhere. The air of these flasks has deposited its dust, germs and all, and is practically free from suspended matter.

I had a chamber erected, the lower half of which is of wood, its upper half being enclosed by four glazed window-frames. It tapers to a truncated cone at the top. It measures in plan 3 ft. by 2 ft. 6 in, and its height is 5 ft. 10 in. On February 6 it was closed, every crevice that could admit dust, or cause displacement of the air, being carefully pasted over with paper. The electric beam at first revealed the dust within the chamber as it did in the air of the laboratory. The chamber was examined almost daily; a perceptible diminution of the floating matter being noticed as time advanced. At the end of a week the chamber was optically empty, exhibiting no trace of matter competent to scatter the light. Such must have been the case in the stagnant caves of the Paris Observatory. Were our electric beam sent through the air of these caves its track would be invisible; thus showing the indissoluble association of the scattering of light by air and its power to generate life.

I will now turn to what seems to me a more interesting application of the luminous beam than any hitherto described. My reference to Professor Lister's interpretation of the fact, that air which has passed through the lungs cannot produce putrefaction, is fresh in your memories. 'Why air,' said he, 'introduced into the pleural cavity, through a wounded lung, should have such wholly different effects from that entering through a permanently open wound, penetrating from without, was to me a complete mystery, till I heard of the germ theory of putrefaction, when it at once occurred to me that it was only natural that the air should be filtered of germs by the air passages, one of whose offices is to arrest inhaled particles of dust, and prevent them from entering the air-cells.'

Here is a surmise which bears the stamp of genius, but which needs verification. If, for the words 'it is only natural' we were authorised to write 'it is perfectly certain,' the demonstration would be complete. Such demonstration is furnished by experiments with a beam of light. One evening, towards the close of 1869, while pouring various pure gases across the dusty track of a luminous beam, the thought occurred to me of using my breath instead of the gases. I then noticed, for the first time, the extraordinary darkness produced by the expired air, towards the end of the expiration. Permit me to repeat the experiment in your presence. I fill my lungs with ordinary air and breathe through a glass tube across the beam. The condensation of the aqueous vapour of the breath is shown by the formation of a luminous white cloud of delicate texture. We abolish this cloud by drying the breath previous to its entering the beam; or, still more simply, by warming the glass tube. The luminous track of the beam is for a time uninterrupted by the breath, because the dust returning from the lungs makes good, in great part, the particles displaced. After a time, however, an obscure disk appears in the beam, the darkness of which increases, until finally, towards the end of the expiration, the beam is, as it were, pierced by an intensely black hole, in which no particles whatever can be discerned. The deeper air of the lungs is thus proved to be absolutely free from suspended matter. It is therefore in the precise condition required by Professor Lister's explanation. This experiment may be repeated any number of times with the same result. I think it must be regarded as a crowning piece of evidence both of the correctness of Professor Lister's views and of the impotence, as regards vital development, of optically pure air. [Footnote: Dr. Burden Sanderson draws attention to the important observation of Brauell, which shows that the contagium of a pregnant animal, suffering from splenic fever, is not found in the blood of the foetus; the placental apparatus acting as a filter, and holding back the infective particles.]

Application of Luminous beams to Water.

The method of examination here pursued is also applicable to water. It is in some sense complementary to that of the microscope, and may, I think, materially aid enquiries conducted with that instrument. In microscopic examination attention is directed to a small portion of the liquid, and the aim is to detect the individual particles. By the present method a large portion of the liquid is illuminated, the collective action of the particles being revealed, by the scattered light. Care is taken to defend the eye from the access of all other light, and, thus defended, it becomes an organ of inconceivable delicacy. Indeed, an amount of impurity so infinitesimal as to be scarcely expressible in numbers, and the individual particles of which are so small as wholly to elude the microscope, may, when examined by the method alluded to, produce not only sensible, but striking, effects upon the eye.

We will apply the method, in the first place, to an experiment of M. Pouchet intended to prove conclusively that animalcular life is developed in cases where no antecedent germs could possibly exist. He produced water from the combustion of hydrogen in air, justly arguing that no germ could survive the heat of a hydrogen flame. But he overlooked the fact that his aqueous vapour was condensed in the air, and was allowed as water to trickle through the air. Indeed the experiment is one of a number by which workers like M. Pouchet are differentiated from workers like Pasteur. I will show you some water, produced by allowing a hydrogen flame to play upon a polished silver condenser, formed by the bottom of a silver basin, containing ice. The collected liquid is pellucid in the common light; but in the condensed electric beam it is seen to be laden with particles, so thick-strewn and minute as to produce a continuous luminous cone. In passing through the air the water loaded itself with this matter; and the deportment of such water could obviously have no influence in deciding this great question.

We are invaded with dirt not only in the air we breathe, but in the water we drink. To prove this I take the bottle of water intended to quench your lecturer's thirst; which, in the track of the beam, simply reveals itself as dirty water. And this water is no worse than the other London waters. Thanks to the kindness of Professor Frankland, I have been furnished with specimens of the water of eight London companies. They are all laden with impurities mechanically suspended. But you will ask whether filtering will not remove the suspended matter? The grosser matter, undoubtedly, but not the more finely divided matter. Water may be passed any number of times through bibulous paper, it will continue laden with fine matter. Water passed through Lipscomb's charcoal filter, or through the filters of the Silicated Carbon Company, has its grosser matter removed, but it is thick with fine matter. Nine-tenths of the light scattered by these suspended particles is perfectly polarised in a direction at right angles to the beam, and this release of the particles from the ordinary law of polarisation is a demonstration of their smallness. I should say by far the greater number of the particles concerned in this scattering are wholly beyond the range of the microscope, and no ordinary filter can intercept such particles. It is next to impossible, by artificial means, to produce a pure water. Mr. Hartley, for example, some time ago distilled water while surrounded by hydrogen, but the water was not free from floating matter. It is so hard to be clean in the midst of dirt. In water from the Lake of Geneva, which has remained long without being stirred, we have an approach to the pure liquid. I have a bottle of it here, which was carefully filled for me by my distinguished friend Soret. The track of the beam through it is of a delicate sky-blue; there is scarcely a trace of grosser matter.

The purest water that I have seen--probably the purest which has been seen hitherto--has been obtained from the fusion of selected specimens of ice. But extraordinary precautions are required to obtain this degree of purity. The following apparatus has been constructed for this purpose: Through the plate of an air-pump passes the shank of a large funnel, attached to which below the plate is a clean glass bulb. In the funnel is placed a block of the most transparent ice, and over the funnel a glass receiver. This is first exhausted and refilled several times with air, filtered by its passage through cotton-wool, the ice being thus surrounded by pure moteless air. But the ice has previously been in contact with mote-filled air; it is therefore necessary to let it wash its own surface, and also to wash the bulb which is to receive the water of liquefaction. The ice is permitted to melt, the bulb is filled and emptied several times, until finally the large block dwindles to a small one. We may be sure that all impurity has been thus removed from the surface of the ice. The water obtained in this way is the purest hitherto obtained. Still I should hesitate to call it absolutely pure. When condensed light is sent through it, the track of the beam is not invisible, but of the most exquisitely delicate blue. This blue is purer than that of the sky, so that the matter which produces it must be finer than that of the sky. It may be and indeed has been, contended that this blue is scattered by the very molecules of the water, and not by matter suspended in the water. But when we remember that this perfection of blue is approached gradually through stages of less perfect blue; and when we consider that a blue in all respects similar is demonstrably obtainable from particles mechanically suspended, we should hesitate, I think, to conclude that we have arrived here at the last stage of purification. The evidence, I think, points distinctly to the conclusion that, could we push the process of purification still farther, even this last delicate trace of blue would disappear.

Chalk-water. Clark's Softening Process.

But is it not possible to match the water of the Lake of Geneva here in England? Undoubtedly it is. We have in England a kind of rock which constitutes at once an exceedingly clean recipient and a natural filter, and from which we can obtain water extremely free from mechanical impurities. I refer to the chalk formation, in which large quantities of water are held in store. Our chalk hills are in most cases covered with thin layers of soil, and with very scanty vegetation. Neither opposes much obstacle to the entry of the rain into the chalk, where any organic impurity which the water may carry in is soon oxidised and rendered harmless. Those who have scampered like myself over the downs of Hants and Wilts will remember the scarcity of water in these regions. In fact, the rainfall, instead of washing the surface and collecting in streams, sinks into the fissured chalk and percolates through it. When this formation is suitably tapped, we obtain water of exceeding briskness and purity. A large glass globe, filled with the water of a well near Tring, shows itself to be wonderfully free from mechanical impurity. Indeed, it stands to reason that water wholly withdrawn from surface contamination, and percolating through so clean a substance, should be pure. It has been a subject much debated, whether the supply of excellent water which the chalk holds in store could not be rendered available for London. Many of the most eminent engineers and chemists have ardently recommended this source, and have sought to show, not only that its purity is unrivalled, but that its quantity is practically inexhaustible. Data sufficient to test this are now, I believe, in existence; the number of wells sunk in the chalk being so considerable, and the quantity of water which they yield so well known.

But this water, so admirable as regards freedom from mechanical impurity, labours under the disadvantage of being rendered very hard by the carbonate of lime which it holds in solution. The chalk-water in the neighbourhood of Watford contains about seventeen grains of carbonate of lime per gallon. This, in the old terminology, used to be called seventeen degrees of hardness. This hard water is bad for tea, bad for washing, and it furs our boilers, because the lime held in solution is precipitated by boiling. If the water be used cold, its hardness must be neutralised at the expense of soap, before it will give a lather. These are serious objections to the use of chalk-water in London. But they are successfully met by the fact that such water can be softened inexpensively, and on a grand scale. I had long known the method of softening water called Clark's process, but not until recently, under the guidance of Mr. Homersham, did I see proof of its larger applications. The chalk-water is softened for the supply of the city of Canterbury; and at the Chiltern Hills it is softened for the supply of Tring and Aylesbury. Caterham also enjoys the luxury.

I have visited all these places, and made myself acquainted with the works. At Canterbury there are three reservoirs covered in and protected, by a concrete roof and layers of pebbles, both from the summer's heat and the winter's cold. Each reservoir holds 120,000 gallons of water. Adjacent to these reservoirs are others containing pure slaked lime--the so-called 'cream of lime.' These being filled with water, the lime and water are thoroughly mixed by air forced by an engine through apertures in the bottom of the reservoir. The water soon dissolves all the lime it is capable of dissolving. The mechanically suspended lime is then allowed to subside to the bottom, leaving a perfectly transparent lime-water behind.

The softening process is this: Into one of the empty reservoirs is introduced a certain quantity of the clear lime-water, and after this about nine times the quantity of the chalk-water. The transparency immediately disappears--the mixture of the two clear liquids becoming thickly turbid, through the precipitation of carbonate of lime. The precipitate is crystalline and heavy, and in about twelve hours a layer of pure white carbonate of lime is formed at the bottom of the reservoir, with a water of extraordinary beauty and purity overhead. A few days ago I pitched some halfpence into a reservoir sixteen feet deep at the Chiltern Hills. This depth hardly dimmed the coin. Had I cast in a pin, it could have been seen at the bottom. By this process of softening, the water is reduced from about seventeen degrees of hardness, to three degrees of hardness. It yields a lather immediately. Its temperature is constant throughout the year. In the hottest summer it is cool, its temperature being twenty degrees above the freezing point; and it does not freeze in winter if conveyed in proper pipes. The reservoirs are covered; a leaf cannot blow into them, and no surface contamination can reach the water. It passes direct from the main into the house tap; no cisterns are employed, and the supply is always fresh and pure. This is the kind of water which is supplied to the fortunate people of Tring, Caterham, and Canterbury.

*****

The foregoing article, as far as it relates to the theory which ascribes epidemic disease to the development of low parasitic life within the human life, was embodied in a discourse delivered before the Royal Institution in January 1870. In June 1871, after a brief reference to the polarisation of light by cloudy matter, I ventured to recur to the subject in these terms: What is the practical use of these curiosities? If we exclude the interest attached to the observation of new facts, and the enhancement of that interest through the knowledge that facts often become the exponents of laws, these curiosities are in themselves worth little. They will not enable us to add to our stock of food, or drink, or clothes, or jewellery. But though thus shorn of all usefulness in themselves, they may, by carrying thought into places which it would not otherwise have entered, become the antecedents of practical consequences. In looking, for example, at our illuminated dust, we may ask ourselves what it is. How does it act, not upon a beam of light, but upon our own bodies? The question then assumes a practical character. We find on examination that this dust is mainly organic matter--in part living, in part dead. There are among it particles of ground straw, torn rags, smoke, the pollen of flowers, the spores of fungi, and the germs of other things. But what have they to do with the animal economy? Let me give you an illustration to which my attention has been lately drawn by Mr. George Henry Lewes, who writes to me thus:

'I wish to direct your attention to the experiments of von Recklingshausen should you happen not to know them. They are striking confirmations of what you say of dust and disease. Last spring, when I was at his laboratory in Wuerzburg, I examined with him blood that had been three weeks, a month, and five weeks, out of the body, preserved in little porcelain cups under glass shades. This blood was living and growing. Not only were the Amoeba-like movements of the white corpuscles present, but there were abundant evidences of the growth and development of the corpuscles. (I also saw a frog's heart still pulsating which had been removed from the body I forget how many days, but certainly more than a week). There were other examples of the same persistent vitality, or absence of putrefaction. Von Recklingshausen did not attribute this to the absence of germs--germs were not mentioned by him; but when I asked him how he represented the thing to himself, he said the whole mystery of his operation consisted in keeping the blood free from dirt. The instruments employed were raised to a red heat just before use; the thread was silver thread and was similarly treated; and the porcelain cups, though not kept free from air, were kept free from currents. He said he often had failures, and these he attributed to particles of dust having escaped his precautions.'

Professor Lister, who has founded upon the removal or destruction of this 'dirt' momentous improvements in surgery, tells us the effect of its introduction into the blood of wounds. The blood would putrefy and become fetid; and when you examine more closely what putrefaction means, you find the putrefying substance swarming with infusorial life, the germs of which have been derived from the atmospheric dust.

We are now assuredly in the midst of practical matters; and with your permission I will refer once more to a question which has recently occupied a good deal of public attention. As regards the lowest forms of life, the world is divided, and has for a long time been divided, into two parties, the one affirming that we have only to submit absolutely dead matter to certain physical conditions, to evolve from it living things; the other (without wishing to set bounds to the power of matter) affirming that, in our day, life has never been found to arise independently of pre-existing life. I belong to the party which claims life as a derivative of life. The question has two factors--the evidence, and the mind that judges of the evidence; and it may be purely a mental set or bias on my part that causes me throughout this long discussion, to see, on the one side, dubious facts and defective logic, and on the other side firm reasoning and a knowledge of what rigid experimental enquiry demands. But, judged of practically, what, again, has the question of Spontaneous Generation to do with us? Let us see. There are numerous diseases of men and animals that are demonstrably the products of parasitic life, and such diseases may take the most terrible epidemic forms, as in the case of the silkworms of France, referred to at an earlier part of this article. Now it is in the highest degree important to know whether the parasites in question are spontaneously developed, or whether they have been wafted from without to those afflicted with the disease. The means of prevention, if not of cure, would be widely different in the two cases.