Part 2

They May Be Used as “Tracers”

Man’s attempts to describe the universe consist of finding answers to the questions he puts to Nature:

How deep is a well? Toss in a rock. Where is the cat? Hang a bell on him. How far does a wild duck fly? Put a marker on his leg. Where are the fireflies? Just watch at dusk. Is our satellite still up? Listen for the radio signal.

Other questions arise in agricultural research:

How fast do roots grow? How deep? How soon does water get to them after a rain? When does a mouthful of hay reach a cow’s stomach? How long until nutrients get into her blood? Her milk? How far will pine pollen travel on the wind? How deep does an earthworm burrow?

To answer these questions, scientists need some kind of miniature genie, one who will shout at the proper moment, “I’m here!” When the root has reached the fertilizer or the water has reached the root; when the hay becomes transmuted to milk, or the earthworm arrives at a particular spot—then this invisible little servant who has made the trip could announce, “I’m here!”

Such a helpful genie exists as the radioactive atom: he is invisibly small, obedient, transportable, digestible, immune to fire, flood, or famine, able to travel under his invisible cloak to the secret hiding places of Nature’s creatures and announce to waiting Geiger tubes, “I’m here!”

The physically unstable radioactive atom behaves chemically exactly like its stable counterpart until the instant it emits its radiation and becomes stable. For example, radioactive phosphorus behaves, biologically and chemically, like stable phosphorus until it emits a beta particle and becomes stable sulfur. If the beta particle enters a gas-filled Geiger tube, it produces a tiny burst of electrical energy which is registered by the counter.

Like fireflies which reveal themselves at dusk by flashes of light, radioisotopes announce their numbers and locations to sensitive Geiger tubes by flashes of invisible “light.”

How Effective Are Radioactive Tracers?

One way to see how valuable radioactive tracers are is to compare them to standard chemical techniques. A sensitive chemical test can perceive molecules as dilute as 10⁻⁷; that is, it can detect a molecule surrounded by 10 million molecules of another kind. A good radioactive tracer technique, by comparison, can distinguish concentrations of 10⁻¹¹; that is, it can trace one in 100 billion.

In other words by the chemical test you could find a person in metropolitan New York with a secret tattoo on the roof of his mouth. By the tracer method you could find this same person anywhere in the world, even if the world population were multiplied fiftyfold.

In the chemical test you could distinguish the equivalent of one kernel of corn in one-tenth of a boxcar load; in the tracer, one kernel in 850 boxcars.

Plant Nutrition and Metabolism

Most studies of plant nutrition and metabolism pertain to the following questions. What do plants need for their best growth? How do they take in the materials they need? What things are absorbed by roots and what things by foliage? How does the plant turn water and other simple compounds into carbohydrates and proteins?

Specific problems that atomic energy has helped to solve are listed.

What Happens to Fertilizer in the Soil?

Early research indicated that only 10 to 12 per cent of phosphorus fertilizers was taken up by plants in the first year; the rest was “locked into” the soil or washed away. With radioactive phosphorus-32 scientists found that as much as 50 to 70 per cent of the phosphorus in a plant came from the fertilizer during the first two or three weeks of growth.

Do Plants Absorb Through Roots Only?

Fertilizer applied to soil is largely wasted because it is either bound by soil particles or is washed out of the root zone. If chemical elements could go directly into leaves and bypass the wastefulness of soils, a tremendous saving would result.

Botanists have learned in recent years that the foliage of plants can take in some nutrients much as roots can. With tracers they discovered that many nutrients are readily taken up by foliage, including bark of dormant trees, even at temperatures below freezing. As shown by isotopic tracers, elements such as phosphorus, nitrogen, and potassium move both up and down from the point of application at rates similar to those following root absorption. Urea (a nitrogen compound) is now used as a nutrient foliar spray for many fruit and vegetable crops in this country.

Where Should Fertilizer Be Placed?

Even before the use of tracers, agronomists realized the inefficiency of spreading fertilizer uniformly over a seed-bed. They know the fertilizer should be placed somewhere near the seed, but where? Above? Below? Beside? Below and beside? How far away? They had conducted some research, but the methods were slow and tedious.

Using tracers, the researchers confirmed earlier findings that roots within two or three days reached fertilizer placed less than two inches directly below seeds, but the roots tended to congregate there. When the fertilizer was two inches below and two inches to the side, roots reached it within a week and a better root system developed. With three inches between seeds and fertilizer, the desired seedling “boost” was delayed three or four weeks. (See Fig. 1.)

Do Fertilizers Move Fast in Plants?

The movement of radioactive phosphorus from root to leaf was found to be remarkably fast, sometimes requiring less than twenty minutes. (See Fig. 2.)

What Else Do Radioisotopes Tell Us?

Some plants take in chemicals that the plant probably cannot use: for example, the so-called locoweeds accumulate enormous amounts of selenium. With tracer techniques, we can see that the root uptake process has poor powers of discrimination.