Part 4

Canine length, 0.75" very worn 0.82" 0.85" lower (19 mm) (21 mm) (21 mm)

Testes ---- ---- 0.5 × 1.0" 0.5 × 0.75" (13×25 mm) (13×19 mm)

Teats Tiny, not Dark, ---- ---- apparent evident ----------------------------------------------------------------------

Gross subjective estimates based on tooth wear

Estimated

On March 5, 1974, the wolves were again immobilized for pre-release processing in Minnesota. An initial dose, and several supplemental doses of phencyclidine and promazine were administered intramuscularly and intraperitoneally between 9:00 a.m. and 4:30 p.m. CDT. The wolves were restrained with muzzles and their legs were bound together during processing and transport. Two of the wolves were blindfolded because they were too active otherwise.

The wolves were ear-tagged with both Minnesota and Michigan Department of Natural Resources tags, and weights and body measurements were taken (Fig. 6, 7). Their teeth were inspected and canines were measured to try to obtain an indication of age. Each animal was fitted with a radio transmitter (AVM Instrument Co., Champaign, Illinois) molded into an acrylic collar (Mech, 1974).

Each wolf was injected with 1,200,000 units of Bicillin (Wyeth), 2 cc of distemper-hepatitus-leptospirosis vaccine (BioCeutic Laboratories D-Vac HL), 0.5 cc of vitamins A, D, E, (Hoffman-LaRoche Injacom 100), 1 cc of vitamin C-fortified vitamin B complex (Eli-Lilly, Betalin Complex FC), and 2 cc anti rabies vaccine (Fromms Raboid). These injections (Fig. 8) were given to insure that the wolves would be as healthy as possible upon release, and would not contract or introduce diseases in the release area.

Mention of trade names does not constitute endorsement by the U. S. Government.

Some 30 to 60 cc of blood were drawn from each wolf for analysis of its physical condition (Seal et al. 1975).

The processing of the wolves took from 8:45 a.m. to 2:00 p.m. CDT on March 5, 1974. The animals were then transported by truck to International Falls, loaded on an airplane (Fig. 9), and flown for 2 hours (Fig. 10) to the Marquette County Airport, Michigan. They were turned on different sides each half hour while drugged during their processing and transport to prevent lung congestion. At the Marquette Airport they were transferred by van to a 25 foot by 25 foot by 12 foot (7.6 m × 7.6 m × 3.7 m) holding pen on the Huron Mountain Club property 35 miles (56.3 km) northwest of Marquette.

The wolves were released individually into the holding pen while each was still partly under sedation (Fig. 11). The transmitting frequency of each wolf's collar was rechecked on the receiver as each wolf was released into the pen (Fig. 12). All wolves were in the pen by 10:00 p.m. EDT, and were held there until March 12.

Four road-killed deer carcasses, provided by the Michigan Department of Natural Resources, had been placed inside the pen for food (Fig. 13), and a tub of drinking water was provided. Carcasses of five road-killed deer and a black bear (Ursus americanus) were placed within a half-mile (0.8 km) of the release pen as food for the wolves after their release.

We had scheduled the release for mid-March for several reasons which we felt would maximize chances for success. Deer are concentrated then in the Huron Mountain area and vulnerable to predation. Pregnancy and subsequent whelping of the alpha female might increase her attachment to the new area. Furthermore, the snow is usually deepest then and hinders travel. However, a few days before the release, a freak rainstorm had settled the snow, and cold temperatures had frozen it so hard that animals could walk readily on top, making travel conditions excellent.

An observation shack 120 feet (36.6 m) from the pen was used to determine the activities and interactions of the four wolves. Weise spent three nights in the shack and also observed the wolves each day of the one-week penned period, for a total of 20 hours of observation (Fig. 14).

During preliminary air and ground checks of radio equipment, we discovered that Wolf No. 10 had a defective collar. Thus on March 12, we subdued her with a choker, restrained her with ropes, replaced her collar and released her just after sunset. We then opened the pen, and let the other wolves loose.

The subsequent locations of the wolves were then checked intermittently through aerial radio-tracking (Mech 1974), with a receiver and antenna from the AVM Instrument Co., Champaign, Illinois, used in a Cessna 172 and a Piper Colt. We made two flights each day for the first 2 days after release, one each day when weather permitted, until April 20, three per week in May, approximately two per week from June through September, and three per week in October and November. A total of 194 hours were flown, 80 per cent by Weise, and the remainder by Hook. Aerial locations were usually recorded to the nearest 40 acres (16 ha.).

We also tracked the wolves from the ground whenever interesting or significant activities were observed during flights or were reported by ground observers. Carcasses of prey animals were investigated from the ground after consumption was complete and the wolves had left. Deer eaten by the wolves were considered killed by them if the ground check revealed fresh blood or flesh, or signs of a struggle. Scats were collected along the tracks of the wolves in the snow whenever possible.

When radio signals were received from the same location for unusually long periods, ground checks were made to determine the cause.

Attempts were made to verify all sightings and track records reported by local citizens, by comparing them with the aerially-determined locations.

RESULTS

Social Structure of the Translocated Wolves

Wolves No. 11, 12, and 13 were captured in Minnesota within a mile (1.6 km) of each other, and No. 11 and 12 were taken in the same trap set 12 days apart; Wolf No. 10 was caught approximately 7.5 miles (12.1 km) southeast of the others (Table 2). All were judged to be thin but in good condition.

Females No. 10 and 11 were introduced into the same pen on January 23. No. 11 was reluctant to enter the pen containing No. 10 while several observers were around, but entered within 15 minutes after all but one had left. No. 11 went directly to No. 10 which was lying in a corner as she usually did, and pawed the fence at No. 10's back. When the pawing became more vigorous, No. 10 snapped at No. 11, moving only her head and neck. No. 11 then turned directly to No. 10, sniffed the top of her head and mane, and lay down beside No. 10 with her nose still in No. 10's mane. No. 10 remained still throughout the whole process. The trapper reported that later No. 11 licked the face of No. 10. Sniffing and licking anteriorally are usually signs of intimacy between wolves (Schenkel 1947).

The two male wolves (No. 12 and No. 13) were allowed into the pen with the two females on February 4. No. 13 remained in the original adjoining pen and did not move in with the females immediately, but No. 12 did. There were no signs of aggression among any of the four wolves. No. 11, 12, and 13 moved freely around the pen while in Minnesota, but No. 10 most often lay in one corner by herself.

Trapper Himes first observed vaginal bleeding in female 11 on February 7. He observed Wolves 11 and 12 mating (with normal coupling) on February 12 and 16.

No unusual aggressive or agonistic social interactions of consequence were observed among the wolves while penned in Michigan, from March 5 to 12. Animals 11, 12 and 13 would lie down and feed together in various combinations. No. 10 was less active than the others and often stayed inside a shelter box within the enclosure, but would come out and mix with the other wolves for brief periods when humans were not in evidence. Her actions were indicative of a low ranking, immature, distressed, or alien animal.

Male No. 12 was the only wolf that would stare directly at a person approaching the pen. He was bolder and more direct in his actions than any of the other animals. This is the wolf that mated with adult female No. 11 while penned in Minnesota, and thus can be considered the "alpha male," or pack leader.